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CAS RN: | Product Number: R0229
Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
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Purity:
Synonyms:
- Griffithsin
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|---|
| 1ML |
$266.00
|
2 | 1 | 19 |
|
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Supplemental Product Information:
[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0229
Product name: Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant GRFT, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 13 kDa
Preservative : None
Stabilizer: None
Description
The rGRFT is a carbohydrate-binding protein (Lectin) derived from Griffithsia sp. The rGRFT gene product is expressed in Escherichia coli. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope. Consequently, rGRFT exhibits a hemagglutination activity to a guinea pig (but not rabbit) reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0234 rGRFT-Biotin [for Manα(1-2)Man]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
| Product Number | R0229 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 10000 to 25000(SDS-PAGE) |
| Hemagglutination activity | max. 16 micro g/mL(pH7.2) |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3504.00.5000 |
Application
Recombinant lectin
References
- Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp
- Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture
- Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding
Product Articles
[TCIMAIL No.188] Recombinant Antiviral Lectin GRFT[TCIMAIL No.193] Hemagglutinin: Recombinant Lectins
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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Analytical Charts
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Other Documents
Related Products
Recombinant Polyporus squamosus lectin (= rPSL1a) expressed in Escherichia coli
| Product # | R0225 |
| CAS RN | |
| Purity / Analysis Method: |
rGRFT-LecBeads [for Manα(1-2)Man]
| Product # | R0239 |
| CAS RN | |
| Purity / Analysis Method: |
D-(+)-Mannose
| Product # | M0045 |
| CAS RN | 3458-28-4 |
| Purity / Analysis Method: | >98.0%(HPLC) |