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Imaging is an indispensable experimental technique for observing and analyzing biological phenomena. TCI offers a wide range of reagents from highly versatile fluorescent labeled probes to clearing reagents for animals and plants.
Tissue Clearing Reagents
Tissue clearing is fast becoming one of the main methods to observe the inner structure of deep tissue. TCI offers a wide range of clearing reagents for use with both animals and plants.
Fluorescence Probes for Detection
We have a wide variety of fluorescent dye-labeled secondary antibodies, as well as fluorescent dyes that are pre-weighed and pre-dissolved in solvent for ease of use.
Lipid Droplet Staining Dyes
Lipid droplets are cellular organelles used to store lipids such as triglycerides and cholesterol. There is growing evidence for their roles in autophagy1) and cellular senescence.2)
ShoyakuGreen (= TFMAQ-8Ph) is a green fluorescent dye (λex, max = 404 nm, λem, max = 473 nm) which not only has a high specificity for fat droplets but also low cytotoxicity.
PC6S emits strong green fluorescence (λex, maxem, max = 535 nm) and can dye clearly not only cellular lipid droplets but also tissue lipid droplets.
Oil red O is a diazo dye which has been widely used for staining fat cell and lipids, and stains low polar lipids red.
- 1) Autophagy regulates lipid metabolism
- 2) Inhibition of endothelial p53 improves metabolic abnormalities related to dietary obesity
Hoechst® is a registered trademark of Hoechst GmbH. MitoTracker® is a registered trademark of Molecular Probes, Inc.
Light in the near-infrared region has high tissue permeability, underlying its application in deep tissue imaging in vivo. TCI offers near-infrared fluorochromes with absorption and emission wavelengths in the near-infrared region, such as Indocyanine Green (ICG). We are also happy to discuss bulk synthesis by request.