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CAS RN: | Product Number: N1109

4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]

  • p-Nitrophenyl Phosphate (Ready-to-use solution)
  • pNPP (Ready-to-use solution)
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Product Information
Volume : 100 mL
Preservative : 0.02% Sodium azide

This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears. After terminating the reaction by adding NaOH solution, the absorbance of reaction product is measured at 405 nm.

The following steps are for a 96-well plate format.
1. Bring pNPP solution (Product No. N1109) to room temperature.
2. Gently mix the pNPP solution (Product No. N1109).
3. Add 100uL of pNPP solution (Product No. N1109) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100 uL of 1 N NaOH (Product No. S0542) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 405 nm.

The product should be stored at 4 ºC and used within 6 months from purchase.The product may be frozen but this does not affect its quality; thaw it at room temperature before use.

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D4005 Disodium 4-Nitrophenyl Phosphate Hexahydrate [for Biochemical Research]
S0542 Sodium Hydroxide (1mol / L in Water)

Product Number N1109
Physical State (20 deg.C) Liquid
Storage Temperature -20°C
Condition to Avoid Heat Sensitive
Appearance Colorless to Light orange to Yellow clear liquid
Alkaline Phosphatase detection test to pass test
Properties (reference)
Pictogram Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H302 : Harmful if swallowed.
H315 : Causes skin irritation.
H318 : Causes serious eye damage.
H361 : Suspected of damaging fertility or the unborn child.
H371 : May cause damage to organs.
H373 : May cause damage to organs through prolonged or repeated exposure.
H334 : May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H351 : Suspected of causing cancer.
H402 : Harmful to aquatic life.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P273 : Avoid release to the environment.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P270 : Do not eat, drink or smoke when using this product.
P202 : Do not handle until all safety precautions have been read and understood.
P285 : In case of inadequate ventilation wear respiratory protection.
P201 : Obtain special instructions before use.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P308 + P313 : IF exposed or concerned: Get medical advice/ attention.
P304 + P341 : IF INHALED: If breathing is difficult, remove person to fresh air and keep comfortable for breathing.
P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
P301 + P312 + P330 : IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth.
P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor.
P405 : Store locked up.
Related Laws:
Transport Information:
HS Number 2919.90.3000
4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]


  • Antibodies, A Laboratory Manual, ed. by E. Harlow, D. Lane, Cold Spring Harbor Laboratory, New York, 1988, p. 597.
  • Enzyme immunoassays in diagnostic medicine
    • A. Voller, D. E. Bidwell, A. Bartlett, Bull. World Health Organ. 1976, 53, 55-65.

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